Empowering your research with mRNA technology
以终为始
首先，我们需要明确mRNA疗法的目标——治疗疾病或改善健康状况，再通过严谨的研究和数据分析，确定需要编码的蛋白质。
蛋白质结构分析
随后，我们选择目标蛋白质。这一步骤包括识别与疾病相关的蛋白质，并深入了解其结构与功能。
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
蛋白质序列优化
接着我们对选定的蛋白质序列进行优化，确保其在人体内的稳定性和功能性。通过计算机模拟与生物信息学工具，我们能预测并改进蛋白质结构以提升疗效。
AUGUUCGUGUUUUUGGUUCUGCUACCACUUGUUUCAUCCCAGUGCGUGAACCUUACAACACGGACGCAGUUGCCGCCUGCGUACACUAAUUCAUUCACGCGAGGGGUGUAUUACCCGGACAAGGUGUUUCGUUCGUCGGUGCUUCACUCCACGCAAGACCUAUUCCUGCCAUUUUUCUCUAAUGUCACGUGGUUUCACGCGAUUCAUGUGAGUGGCACGAAUGGCACUAAACGUUUCGACAACCCGGUAUUACCGUUUAAUGACGGGGUGUACUUCGCCUCGACAGAGAAAUCAAAUAUCAUUAGAGGCUGGAUCUUUGGCACAACACUAGACUCUAAGACGCAAAGCUUGCUUAUUGUAAAUAAUGCUACCAAUGUCGUUAUCAAAGUAUGUGAAUUUCAAUUCUGCAAUGACCCGUUCCUUGGGGUAUAUUAUCAUAAAAAUAAUAAGUCUUGGAUGGAGAGUGAGUUUAGAGUCUAUUCGUCGGCAAACAAUUGUACUUUUGAGUACGUUUCACAACCUUUCUUAAUGGAUCUGGAGGGCAAACAAGGGAAUUUCAAGAACUUGAGGGAGUUUGUGUUCAAGAAUAUUGAUGGCUACUUCAAAAUUUACAGUAAACACACACCGAUUAAUUUAGUACGUGAUCUACCCCAGGGGUUUUCCGCCCUCGAGCCACUCGUGGACCUUCCUAUUGGAAUCAAUAUAACCCGGUUCCAGACAUUGUUAGCCCUUCAUAGGAGCUAUUUAACUCCAGGAGACAGCUCUUCGGGCUGGACAGCGGGAGCGGCAGCGUAUUACGUCGGCUAUUUGCAGCCCCGUACCUUUCUAUUAAAGUACAAUGAGAAUGGCACUAUUACGGAUGCGGUUGAUUGCGCUCUGGACCCCUUGUCCGAAACAAAGUGUACCCUCAAGUCGUUCACUGUCGAAAAGGGCAUCUAUCAGACGAGUAAUUUCCGUGUACAACCGACGGAGUCGAUAGUUCGCUUUCCAAAUAUUACCAAUCUUUGUCCAUUCGGCGAGGUCUUUAACGCAACUCGAUUCGCUUCCGUUUACGCCUGGAACCGGAAACGGAUAAGCAACUGUGUGGCGGACUAUAGCGUCCUGUACAAUUCUGCGAGCUUCUCAACAUUUAAGUGUUAUGGAGUGAGCCCCACUAAAUUGAAUGACCUCUGCUUCACCAAUGUCUAUGCGGACUCCUUUGUAAUCCGGGGUGAUGAGGUACGACAAAUUGCACCCGGCCAGACGGGGAAAAUCGCUGAUUACAAUUAUAAGCUACCGGACGACUUUACCGGCUGCGUAAUAGCGUGGAACUCUAACAACCUCGAUUCUAAGGUGGGUGGGAACUACAACUAUCUGUACAGGCUAUUUCGAAAAUCUAAUCUUAAGCCUUUUGAACGCGAUAUUUCAACUGAAAUUUAUCAGGCCGGGUCAACGCCUUGCAACGGGGUGGAGGGGUUUAAUUGCUAUUUCCCGUUGCAAUCCUACGGCUUUCAGCCGACAAACGGAGUAGGAUACCAACCGUACCGAGUAGUAGUAUUAUCCUUCGAACUUUUACACGCACCGGCGACCGUUUGCGGUCCAAAGAAGUCUACUAAUCUAGUAAAAAACAAAUGUGUUAAUUUCAACUUUAACGGGUUGACUGGCACAGGCGUUCUGACUGAGUCUAACAAAAAGUUCCUGCCGUUUCAACAAUUUGGUAGAGAUAUAGCGGACACCACUGAUGCAGUAAGAGAUCCCCAAACGCUGGAAAUUCUAGACAUCACACCCUGUUCCUUUGGAGGUGUCUCUGUGAUCACUCCCGGGACUAAUACCAGCAAUCAAGUCGCGGUUCUCUACCAGGAUGUCAACUGUACAGAGGUACCGGUAGCAAUCCAUGCUGAUCAGCUAACGCCGACUUGGAGAGUCUACAGCACCGGAUCUAACGUUUUUCAGACGCGGGCUGGGUGUCUAAUAGGCGCGGAGCACGUAAACAAUUCAUAUGAGUGUGAUAUACCCAUCGGUGCCGGGAUCUGCGCUUCAUACCAGACACAAACUAAUAGCCCAAGGCGCGCUCGAUCCGUGGCUAGUCAGAGUAUUAUAGCUUAUACGAUGUCCUUAGGAGCAGAGAAUUCUGUCGCCUAUAGUAACAAUUCAAUUGCUAUCCCUACCAAUUUCACGAUCUCUGUUACUACCGAGAUUUUACCAGUUAGCAUGACCAAAACGAGCGUGGAUUGUACAAUGUAUAUUUGUGGAGACAGCACAGAAUGUUCGAAUCUACUGCUACAGUAUGGCAGUUUUUGCACACAAUUAAAUAGAGCGCUGACAGGCAUUGCCGUGGAACAGGAUAAAAAUACCCAGGAAGUCUUCGCACAAGUAAAGCAAAUUUACAAAACCCCGCCGAUCAAAGAUUUUGGCGGGUUUAAUUUCUCACAGAUACUGCCCGAUCCUAGUAAGCCCAGCAAACGUAGUUUCAUAGAAGACUUGCUCUUCAACAAAGUAACGUUGGCAGAUGCAGGAUUUAUUAAACAAUAUGGUGACUGUCUAGGGGACAUUGCAGCGCGCGAUUUAAUUUGUGCGCAAAAAUUCAACGGACUCACGGUGCUGCCGCCCUUAUUGACGGACGAGAUGAUAGCGCAAUAUACGUCAGCACUCUUGGCGGGGACCAUAACCUCGGGCUGGACCUUCGGCGCCGGAGCCGCGUUGCAGAUACCAUUUGCUAUGCAAAUGGCGUAUAGAUUCAACGGAAUCGGGGUAACUCAAAACGUUCUUUAUGAAAACCAGAAACUGAUCGCCAACCAGUUCAAUAGCGCCAUUGGCAAAAUACAAGACAGCUUGUCAUCAACAGCCUCUGCACUGGGUAAAUUACAGGAUGUUGUAAACCAGAAUGCGCAAGCUUUAAACACUCUUGUGAAACAACUCUCGUCAAAUUUCGGGGCUAUCAGCUCUGUGCUCAAUGACAUACUCUCGAGACUUGAUAAGGUGGAGGCAGAAGUACAAAUUGAUAGGCUGAUUACUGGUCGUCUCCAAUCCCUUCAAACUUAUGUUACUCAGCAACUGAUCAGAGCAGCUGAGAUUCGUGCAUCGGCGAACCUGGCAGCUACUAAGAUGUCAGAGUGCGUCUUAGGCCAGUCAAAGCGGGUGGACUUCUGUGGGAAAGGCUACCACCUCAUGUCAUUCCCCCAGUCAGCUCCACAUGGCGUGGUUUUCUUACAUGUGACGUACGUUCCAGCGCAGGAGAAAAAUUUCACAACAGCUCCAGCCAUAUGUCAUGAUGGUAAGGCGCACUUCCCUCGGGAAGGCGUCUUUGUCUCUAACGGCACUCAUUGGUUUGUGACCCAGCGCAAUUUUUAUGAACCUCAAAUAAUAACAACGGAUAACACGUUCGUGAGCGGAAAUUGUGAUGUGGUGAUCGGCAUAGUGAACAACACAGUAUACGACCCUCUCCAACCGGAACUCGAUUCCUUCAAAGAAGAACUUGACAAAUACUUUAAAAACCAUACCUCCCCAGAUGUAGACCUUGGUGACAUAUCUGGUAUUAAUGCAUCCGUGGUUAACAUACAGAAAGAAAUAGAUCGACUUAACGAGGUGGCUAAGAACUUAAACGAAUCCCUCAUCGACCUCCAAGAGUUAGGAAAGUACGAACAGUACAUCAAGUGGCCAUGGUAUAUUUGGUUAGGAUUCAUCGCUGGACUCAUAGCUAUCGUUAUGGUGACAAUAAUGCUGUGCUGCAUGACGUCGUGUUGUUCUUGCUUAAAGGGAUGUUGUUCCUGCGGUUCUUGUUGCAAAUUUGAUGAGGACGACUCAGAGCCCGUGCUUAAGGGCGUUAAGCUGCAUUACACGUAG
mRNA序列优化
为确保mRNA在体内的稳定性和高效翻译，我们需要对其序列的各个组成部分进行优化。这些关键元件包括5‘端帽子（5’ Cap）、5‘非翻译区（5’ UTR）、编码序列（CDS）、3‘非翻译区（3’ UTR）和 PolyA尾。每一个元件都扮演着独特的角色，其优化策略也各不相同。
mRNA合成
优化后的mRNA合成过程需要高精度的仪器和严格的质量控制措施，以确保生产的mRNA具有高纯度和活性。
递送
为了保护mRNA并促进其进入细胞，我们将其包裹在脂质纳米颗粒（LNPs）中。这些纳米颗粒有效地逃避了免疫系统的检测，并安全地将mRNA递送到靶细胞。
mRNA释放
一旦进入细胞，被包封的mRNA被释放到细胞质中，通过细胞翻译机制产生目标蛋白。这一步对mRNA药物的治疗效果至关重要。
蛋白质的功能
最终，翻译后的蛋白质开始在细胞内发挥其生物学功能。无论是修复受损细胞，调节免疫反应，还是抑制病原体增殖，这些蛋白质都致力于实现治疗效果、为患者健康服务。